By narrowly winning more than 50% of the vote Mr Mnangagwa avoided a run-off election against Mr Chamisa.
Advocate Thabani Mpofu, head of a legal team that is mounting the MDC Constitutional Court petition, vowed Wednesday "the poll challenge is going ahead, prepared, clear and convincing".
It's amusing that Chamisa (MDC Alliance presidential candidate Nelson) entered an election wanting to win only.
The latest developments mean the planned inauguration of President-elect Emmerson Mnangagwa, can not go ahead today as scheduled as the court has to deal with the matter first.
Former minister of education David Coltart added his voice to confirm the filing of the court papers to challenge the presidential results and some constituencies. The opposition said the result was rigged.
But the legal challenge to his victory is likely to delay his inauguration that was scheduled to take place on Sunday.
Wear Loose Underwear To Improve Your Sperm Production
Harry Fisch, a clinical professor of urology and reproductive medicine at the Weill Cornell Medical College in New York City. Men's underwear choice can affect their sperm count and quality, according to a new study by Harvard researchers.
The MDC Alliance has a tall order as it has to demonstrate to the court that, indeed, there were instances in which the law was not complied with and show how such irregularities influenced the final results of the elections.
Analysts say that the legal challenge has little chance of success given the courts' historic tilt towards ZANU-PF, which has ruled since independence from British colonial rule in 1980.
"In the alternative, we seek that there be another election which complies with the dictates of the law", Mpofu said.
Mr Chamisa wants the court to declare him the victor or call a fresh election, his lawyer told Reuters news agency on Friday.
He faces charges of inciting the protests last week by proclaiming victory for the opposition.
In a joint statement, the EU, US, Canadian and Australian missions to Zimbabwe urged authorities to guarantee Biti's safety and human rights.
Tendai Biti was released earlier today following my intervention.