Honoring his early film, which starred Jackson, he told audiences to "do the right thing".
"First of all, Spike, I'm so glad you're sitting down", Jackson said. Let us all mobilise, let's all be on the right side of history.
Lee won the award for Best Adapted Screenplay for BlacKkKlansmen and gave an empowering speech about love and equality. As PEOPLE notes, The Daily Show anchor Trevor Noah shared a photo of Lee at the Oscars, watching him scribble down his speech.
The speech was not something Lee had planned, even though some pundits predicted he would finally win an Oscar Sunday.
University of Kansas professor Kevin Willmott has won an Oscar for his contribution to the film "BlacKkKlansman".
Buhari inspects who his wife voted for, Twitter reacts
Mahmood Yakubu, chairman of the INEC, said that 72.8 million cards had been collected out of 84 million registered voters. Acting Inspector General of Police Mohammed Adamu said police would ensure security at the polls.
But true to form, the Oscars came back to their real nature, which is to rip Trump or those on the Right and push their political leanings, when "BlackKklansman" director Spike Lee took the stage.
While the "Best Director" and "Best Picture" categories are to be decided later this evening (February 24), the win comes after almost 20 years of waiting.
John David Washington stars as Stallworth, while Adam Driver, Laura Harrier and Topher Grace also feature in the film. They also won the British Academy Film and Television Arts award, also known as BAFTA, in the same category earlier this month.
Lee received the award from Samuel L. Jackson, who has appeared in Lee's films "Do the Right Thing", "Jungle Fever", "School Daze" and "Mo' Better Blues".
Lee's other films include Malcolm X, She's Gotta Have It, Miracle at St. Anna, Inside Man and 25th Hour.