No topic warnings, the Alex sailed to the port on Saturday resulting from of "intolerable hygienic stipulations" aboard.
Italian Interior Minister Matteo Salvini had tweeted that the Alex should take them to Tunisia and there was no immediate confirmation that Italy had agreed to take in 55 migrants from Malta as announced by the government in Valletta.
Italian populist Salvini last month issued a decree that would bring fines of up to 50,000 euros ($57,000) for the captain, owner and operator of a vessel "entering Italian territorial waters without authorisation".
Mediterranea tweeted that its exhausted crew were living through a "surreal situation", and prolonging the wait was "an unnecessary cruelty".
Seehofer, who himself has taken a restrictive line on migration, already has said Germany would be prepared to take some migrants on board two ships now just outside Italian waters, one of them a German group's, if other European countries do.
"I do not authorise any landing for those who couldn't care less about Italian laws and help the people smugglers", Salvini tweeted late Saturday.
They applauded as they docked on Saturday afternoon.
All 65 were transferred to a Maltese navy ship on Sunday evening, the German charity Sea-Eye which operates the boat said in a statement, adding that its vessel had been refused entry to Valetta port.
And a poll printed within the Italian newspaper Corriere Della Sera on Saturday acknowledged 59% of Italians approved of Mr Salvini shutting Italy's ports off to NGO-crawl vessels. "We are waiting for Italian or Maltese naval arrangements to take these people on board", Mediterranea's Alessandra Sciurba said on Twitter.
"It doesn't seem that the majority of European countries want to do it", said Salvini, vowing to change rules he says place an unfair burden on Italy.
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Italy has been one in every of the main locations for migrants making an try to attain Europe via the North Africa route, mostly from Libya.
Relations between Italy's government and charity rescue ships grew increasingly tense last week, when Sea-Watch 3 captain Carola Rackete was arrested, after forcefully docking her ship in Italy.
The ship's captain Carola Rackete was arrested and accused of endangering police lives at sea.
A purchase ordered her freed, though she quiet faces separate prices of helping other folks smugglers and resisting authorities.
Italy has insisted that the Libyan coast guard intervene and the migrants be taken back to Libya.
Since yesterday there are 65 people on board the "Alan Kurdi" again and again we see how Italy closes its ports.
Salvini ruled out the possibility that the migrants on board the Alan Kurdi will arrive in Italy.
Earlier this week, migrants being held in a detention centre - which is where many returned migrants find yourself - had been killed in an attack which hit the constructing they had been in.
The World Group for Migration (IOM) estimates that 681 folk include died in the Mediterranean Sea to this level in 2019 - 426 of them in the central region between Libya and Tunisia and Italy.